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lambdagt10

規格:

價格:

編號:B234192

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

lambdagt10

商品貨號

B234192

Designations

lambdagt10

Depositors

Monsanto Co., RW Davis, PD Kelly, Monsanto Co.

培養基

U.S. Patent

4,940,835

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

Biosafety Level

Host

Escherichia coli BNN93 (ATCC^® 47024™) (vector propagation); Escherichia coli BNN102 (ATCC^® 47025™) (screen for recombinants)

Vector Information

Size (kb): 43.34

Vector: lambdagt10 (phage, lambda - replacement)

Construct size (kb): 43.34

Cloning sites: EcoRI

Cloning capacity: 7.6 kb

Host range: Escherichia coli

Features:
replicon: lambda

Applications

Vector for constructing cDNA libraries

Comments

The EcoRI site divides the vector into a left arm of 32.7 kb and a right arm of 10.7 kb.

Useful for cloning EcoRI compatible DNA fragments that range between 0-7 kb.

References

Huynh TV, et alConstructing and screening cDNA libraries in lambdagt10 and lambdagt11In: Huynh TV, et alDNA cloning techniques: a practical approach1OxfordIRL Presspp. 49-78, 1985

Sambrook J, et al. Molecular cloning: a laboratory manual. 1Cold Spring Harbor, NY: CSHL Press; 1989.