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pcD(SRalpha)-F115 [moIL-10 cDNA]

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編號:B234275

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

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凍干粉

斜面

甘油

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產品名稱

pcD(SRalpha)-F115 [moIL-10 cDNA]

商品貨號

B234275

Designations

pcD(SRalpha)-F115 [moIL-10 cDNA]

GenBank Number

M37897

培養基

Species

Mus musculus, mouse

Depositors

DNAX Research Institute of Molecular and Cellular Biology, Inc., KW Moore, DNAX Research Institute of Molecular and Cellular Biology, Inc.

Applications

in another host, produces protein interleukin 10

Vector

Construct size (kb): 4.900000095367432

Insert

DNA: cDNA

Insert lengths(kb): 1.340999960899353

Tissue: T-lymphocyte D10 cell line, conconavalin A-treated

Gene product: interleukin 10 [Il10]

Target Gene: interleukin 10

Insert Size (kb)

1.341

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

Restriction digests of the clone give the following sizes (kb): BamHI--3.4, 1.5; EcoRI--4.9; PstI--2.1, 1.55, 0.65, 0.4, <0.3; HindIII--3.1, 1.8; SstI--3.9, 0.6, 0.4.

Contains the complete coding sequence (nt 76-612).

The fragment excised by BamHI consists of the insert plus some vector sequence.

The vector is modified from pcD, with a promoter constructed from the SV40 early promoter and the R segment and U5 sequence of the long terminal repeat of human T-cell leukemia virus type 1 (HTLV-1 LTR).

Classification

Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod

References

Mosmann TR, et al. Nucleic acids encoding cytokine synthesis inhibitory factor (interleukin-10). US Patent 5,231,012 dated Jul 27 1993

Moore KW, et al. Homology of cytokine synthesis inhibitory factor (IL-10) to the Epstein-Barr virus gene BCRFI. Science 248: 1230-1234, 1990. PubMed: 2161559

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

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