| 產品名稱 |
pcD(SRalpha)-F115 [moIL-10 cDNA] |
| 商品貨號 |
B234275 |
| Designations |
pcD(SRalpha)-F115 [moIL-10 cDNA] |
| GenBank Number |
M37897
|
| Species |
Mus musculus, mouse |
| Depositors |
DNAX Research Institute of Molecular and Cellular Biology, Inc., KW Moore, DNAX Research Institute of Molecular and Cellular Biology, Inc. |
| Applications |
in another host, produces protein interleukin 10 |
| Vector |
Construct size (kb): 4.900000095367432 |
| Insert |
DNA: cDNA Insert lengths(kb): 1.340999960899353 Tissue: T-lymphocyte D10 cell line, conconavalin A-treated Gene product: interleukin 10 [Il10] Target Gene: interleukin 10 |
| Insert Size (kb) |
1.341 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--3.4, 1.5; EcoRI--4.9; PstI--2.1, 1.55, 0.65, 0.4, <0.3; HindIII--3.1, 1.8; SstI--3.9, 0.6, 0.4. Contains the complete coding sequence (nt 76-612). The fragment excised by BamHI consists of the insert plus some vector sequence. The vector is modified from pcD, with a promoter constructed from the SV40 early promoter and the R segment and U5 sequence of the long terminal repeat of human T-cell leukemia virus type 1 (HTLV-1 LTR). |
| Classification |
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References |
Mosmann TR, et al. Nucleic acids encoding cytokine synthesis inhibitory factor (interleukin-10). US Patent 5,231,012 dated Jul 27 1993
Moore KW, et al. Homology of cytokine synthesis inhibitory factor (IL-10) to the Epstein-Barr virus gene BCRFI. Science 248: 1230-1234, 1990. PubMed: 2161559
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
