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pJW101
pJW101
規(guī)格:
價格:
編號:B235023
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pJW101
商品貨號
B235023
Designations
pJW101
GenBank Number

J00153

培養(yǎng)基
Species
Homo sapiens, human
Depositors
BG Forget
Vector
Construct size (kb): 6.349999904632568
Insert
DNA: cDNA
Insert lengths(kb): 0.6000000238418579
Gene product: hemoglobin, alpha 1( alpha 1 globin, globin, alpha 1) [HBA1]
Insert Size (kb)
0.600
Media
ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--similar to uncut; HindIII--5.8, 0.65; BamHI--6.4; AvaI--4.7, 1.65; SmaI--uncut.
The actual cDNA insert is about 600 bp. It cannot be excised as such because of AT tailing, but can be excised as a 1.6 kb MboII fragment containing 845 bp of pMB9.
HindIII and MboII patterns of ATCC preparation match that of Forget's isolate.
HindIII site 275 bp 3' to AT/exon 1 joint (in exon 2); 0.7 kb HindIII fragment contains 3' end of gene; 5.2 kb fragment contains 5' end; TaqI site 130 bp 5' to HindIII site.
The 5' end at the insert coincides exactly with the ATG initiation site, 275 bp from the internal HindIII site. It lacks all the 5' untranslated sequences and the NcoI site bridging the initiation site.
References

Wainscoat JS, et al. Association of two DNA polymorphisms in the alpha globin gene cluster. Am. J. Hum. Genet. 35: 1086-1089, 1983. PubMed: 6316779

Wilson JT, et al. Insertion of synthetic copies of human globin genes into bacterial plasmids. Nucleic Acids Res. 5: 563-581, 1978. PubMed: 345245

Wilson JT, et al. Nucleotide sequence of the coding portion of human alpha globin messenger RNA. J. Biol. Chem. 255: 2807-2815, 1980. PubMed: 6244294

Bernard G Forget, personal communication

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