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當前位置: 首頁 > ATCC代理 > pcDL-SRalphahCD40L B235215
pcDL-SRalphahCD40L
pcDL-SRalphahCD40L
規格:
價格:
編號:B235215
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pcDL-SRalphahCD40L
商品貨號
B235215
Designations
pcDL-SRalphahCD40L
GenBank Number

L07414

培養基
Species
Homo sapiens, human
Applications
in another host, produces protein CD40 antigen ligand
Vector
Construct size (kb): 3.859999895095825
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pcDL-SRalpha296
Intact vector size: 3.700
Type of vector: plasmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: BamHI
Polylinker sites:
Construction: pcDV1, HTLV-1 LTR
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR
promoter: SV40/HTLV-1 LTR
replicon: pMB1, SV40
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CD40LG
Other gene symbols: CD40L
Gene name: CD40 antigen ligand
Contains complete coding sequence?: Y
Chromosome: X; Localization: X q26
Tissue: CD4-positive T-cells
Type of DNA: cDNA
Insert end: BamHI
Insert end: BamHI
Insert size (kb): 0.9
Cross references: DNA Seq. Acc.: L07414
Insert lengths(kb): 0.8999999761581421
Tissue: CD4-positive T-cells
Gene product: CD40 antigen ligand [CD40L]
Target Gene: CD40 antigen ligand
Insert Size (kb)
0.900
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): HindIII--3.4, 1.5; NcoI--3.5, 1.4; PvuII--4.9; SalI--2.4, 2.5; BamHI--3.9, 1.0.
The insert contains the following restriction sites (approximate kb from the 5' end): HindIII--0.58; NcoI--0.78; PvuII--0.52.
Contains the complete coding sequence (nt 40-825 of the sequence record).
COS-7 cells transfected with this construct demonstrate good surface expression of the protein.
The vector is modified from pcD, with a promoter constructed from the SV40 early promoter and the R segment and U5 sequence of the long terminal repeat of human T-cell leukemia virus type 1 (HTLV-1 LTR).
Subcloned from pBS-hCD40L-6A9 (ATCC 79812/3). The 5' BamHI site is derived from the vector and the 3' end is the naturally occurring BamHI site (nt 929 of the published sequence).
References

Gauchat JF, et al. Human CD40-ligand: molecular cloning, cellular distribution and regulation of expression by factors controlling IgE production. FEBS Lett. 315: 259-266, 1993. PubMed: 7678552

Gauchat JF, et al. Induction of human IgE synthesis in B cells by mast cells and basophils. Nature 365: 340-343, 1993. PubMed: 7690905

Jean-Francois Gauchat, personal communication

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