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JH-5

規(guī)格:

價格:

編號:B235283

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

JH-5

商品貨號

B235283

Designations

JH-5

Species

Homo sapiens, human

培養(yǎng)基

Depositors

H Donis-Keller, JR Howe

Vector

Construct size (kb): 44.90000152587891

Insert

DNA: genomic

Insert lengths(kb): 15.69999980926514

Gene product: DNA Segment, single copy [D12S54]

Alleles: A1, B1, B2, C1, C2, A2

Insert Size (kb)

15.700

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried bacteria-free lysate

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI--20.0, 9.2, 7.0, 6.6, 0.8, 0.7; XbaI--23.0, 8.2, 5.6, 3.8, 3.2, 1.7; BamHI/SalI--20.0, 9.2, 8.6, 6.6; BglII--22.0, 14.0, 7.4, 0.7; Uncut; SalI--20.0, 9.2, 15.7.

Restriction digests of the clone give the following sizes (kb): BamHI/SalI--5.2, 4.8, 4.7 (insert), 20.0, 9.2 (vector).

The BglII and TaqI allele systems are in strong linkage disequilibrium. There is an additional variable BglII fragment at 1.8 kb which may be a dominant polymorphism.

Subcloned from YAC D61E6.

Enzyme(s) not detecting polymorphism: BamHI, EcoRI, HIndIII, MspI, PstI (in a panel of 6 individuals).

References

Howe JR, et al. A new RFLP marker D12S54 maps between F8VWF and KRAS2 on human chromosome 12p. Nucleic Acids Res. 19: 2512, 1991. PubMed: 1675000

Helen Donis-Keller, personal communication

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