黄色小说视频-日本少妇高潮抽搐-国内自拍av-天堂中文资源在线-蜜桃视频在线入口www-91久久综合-亚洲视频在线一区-日日夜夜拍-国产精品资源在线观看-欧美激情国产精品免费-人妻少妇久久中文字幕-成人免费无码大片a毛片-香蕉污视频在线观看-日本电影成人-国内精品久久99人妻无码

歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > pGIKS-BioD B235360
pGIKS-BioD
pGIKS-BioD
規(guī)格:
價格:
編號:B235360
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pGIKS-BioD
商品貨號
B235360
Designations
pGIKS-BioD
GenBank Number

J04423

5597)">J04423 (4947->5597)

培養(yǎng)基
Species
Escherichia coli (Migula) Castellani and Chalmers
Depositors
M Follettie
Applications
For newer versions of the microarray control probes, please see a set of clones genes: ATCC 87840 (kit).
Vector
Construct size (kb): 3.56
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript II KS+
Intact vector size: 2.961
Type of vector: phagemid
Vector end: KpnI
Vector end: SacI
Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR
replicon: pMB1, f1
Cross references:
Vector end (5'): KpnI
Vector end (3'): SacI
Excise insert: XhoI+SmaI
Promoter giving the sense strand: T3
Promoter giving the antisense strand: T7
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Escherichia coli
Gene symbol: bioD
Gene name: dethiobiotin synthetase
Contains complete coding sequence?: N
Type of DNA: genomic
Insert 5' end: XhoI Modification: KpnI linker
Insert 3' end: SmaI Modification: SacI linker
Insert size (kb): 0.66
Cross references: DNA Seq. Acc.: J04423
Nucleotides 1-651 of the insert correspond to
nucleotides 4947-5597 of J04423.
Insert lengths(kb): 0.66
Gene product: dethiobiotin synthetase [bioD]
Insert end (5'): KpnI linker
Insert end (3'): SacI linker
Insert Size (kb)
0.660
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
For newer versions of the microarray control probes, please see a set of clones genes: ATCC 87840 (kit).
The insert contains nt 4947-5597 of Genbank J04423.
The insert contains the following restriction sites (approximate kb from the 5' end): PstI--0.18; PvuII--0.18; RsaI--0.64.
The following restriction enzymes are recommend for linearizing the plasmid for in vitro transcription: T3 = SmaI; T7 = XhoI.
Restriction digests of the clone give the following sizes (kb): SmaI--3.6; SmaI/XhoI--3.0, 0.6; XhoI--3.6.
Classification
Enterobacteriaceae, Escherichia
  • 王經(jīng)理
    手機號：17280875617
    QQ：1438578920
  • 胡經(jīng)理
    手機號：13345964880
    QQ：2438244627
  • 周經(jīng)理
    手機號：17757487661
    QQ：1296385441
  • 于經(jīng)理
    手機號：18067160830
    QQ：2088210172
  • 沈經(jīng)理
    手機號：19548299266
    QQ：2662369050
  • 李經(jīng)理
    手機號：13626845108
    QQ：972239479