91porny首页入口,亚洲免费成人在线,91爱爱视频

YIplac128

規格:

價格:

編號:B235480

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

加入購物車

在線咨詢收藏

產品名稱

YIplac128

商品貨號

B235480

Designations

YIplac128

Depositors

RD Gietz

培養基

Biosafety Level

Vector Information

Size (kb): 4.3020000457763670

Vector: YIplac128 (plasmid)

Promoters: Promoter lac

Construction: pUC19

Marker(s):LEU2,ampR

Construct size (kb): 4.302000045776367

Features: insert detection: lacZ'
marker(s): LEU2
marker(s): ampR
promoter: lac
replicon: pMB1
MCS: HindIII...EcoRI

Applications

YI-type (integrating) shuttle vector

vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase

Comments

Restriction digests of the clone give the following sizes (kb): BglI--2.7, 1.6; PvuI--2.6, 1.7; EcoRI--4.3.

One of 9 shuttle vectors (ATCC 87585 - 87593) allowing lacZ detection of cloned inserts and containing the pUC19 MCS with all 10 cloning sites unique. Vectors differ in mode of replication (YE, YC or YI-type) and yeast marker (LEU2, URA3 or TRP1).

The EcoRI and KpnI restriction sites from the LEU2 gene of S. cerevisiae were removed by using oligo-directed mutagenesis.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

Gietz RD, Sugino A. New yeast Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites. Gene 74: 527-534, 1988. PubMed: 3073106