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Influenza A virus (H1N1)
Influenza A virus (H1N1)
規格:
價格:
編號:B235498
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Influenza A virus (H1N1)
商品貨號
B235498
Classification
Orthomyxoviridae, Influenzavirus A
Deposited As
Influenza A virus
培養基
Agent
Influenza A virus (H1N1)
Strain
A/PR/8/34
Applications
Influenza Research
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format
frozen 1 mL per vial
Storage Conditions
-70°C or colder
Images
ATCC VR-1469 micrograph images
Comments
VR-1469® is a TC-adapted item derived from virus passaged in eggs (ATCC® VR-95™).
Effect on Host
CPE, rounding, degeneration, sloughing
Recommended Host
MDCK cells (ATCC® CCL-34™)
Growth Conditions
Temperature: 35°C
Recommendations for Infection: For best results, infection should be performed on an 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2), optimized for propagation (e.g. MOI 0.01-0.1 recommended). Wash monolayer two times with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 35°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 2-4 days at 35°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.
Name of Depositor
ATCC®, M Coleman, Centers for Disease Control and Prevention
Special Collection
NCRR Contract
Source
Patient in Puerto Rico, 1934
References

Francis T. Immunological relationships of strains of filterable virus recovered from cases of human influenza. Proc. Soc. Exp. Biol. Med. 32: 1172-1175, 1935.

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