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pXPBg Plasmid in Escherichia coli
pXPBg Plasmid in Escherichia coli
規(guī)格:
價(jià)格:
編號(hào):B235526
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pXPBg Plasmid in Escherichia coli
商品貨號(hào)
B235526
Designations
pXPBg Plasmid in Escherichia coli
Depositors
SK Nordeen
培養(yǎng)基
Biosafety Level
1
Vector Information
Size (kb): 5.90
Vector: pXPBg (plasmid)
Promoters: Promoter none
Construction: pSV232AL-Adelta5' derivative
Marker(s):ampR
Construct size (kb): 5.90
Features: insert detection: luciferase
marker(s): ampR
promoter: none
replicon: pMB1
terminator: none
enhancer: none
Applications
 promoter-cloning vector
 shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.4; BglII--6.4.
There is cryptic promoter activity which may be more readily observed with weak promoters. Be careful to do all appropriate controls and map the transcripts.
This is a derivative of plasmids patented by the University of California and is not to be used for commercial purposes without prior arrangement with the University of California.
One of a series of luciferase vectors (ATCC 37574-37584) for assaying promoter activity in mammalian cells.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Nordeen SK. Luciferase reporter gene vectors for analysis of promoters and enhancers. BioTechniques 6: 454-458, 1988. PubMed: 2908509

de Wet JR, et al. Firefly luciferease gene: Structure and expression in mammalian cells. Mol. Cell. Biol. 7: 725-737, 1987. PubMed: 3821727

Steven K Nordeen, personal communication

Shipped
freeze-dried
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