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pNMU-1
pNMU-1
規(guī)格:
價(jià)格:
編號(hào):B235577
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pNMU-1
商品貨號(hào)
B235577
Designations
pNMU-1
Species
Rattus norvegicus, rat
培養(yǎng)基
Depositors
M Barbacid
Vector
Construct size (kb): 11.39999961853027
Insert
DNA: genomic
Insert lengths(kb): 6.800000190734863
Tissue: transformed 3T3 cell line
Gene product: Harvey rat sarcoma viral (v-Ha-ras) oncogene homolog(Harvey rat sarcoma virus oncogene) [HRAS]
Insert Size (kb)
6.800
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Constructed from DNA from NIH 3T3 cell transformed by rat mammary tumor induced by nitroso-methyl urea.
Restriction digests of the clone give the following sizes (kb): BamHI--6.8, 4.5; HindIII--5.6, 3.15, 2.5; EcoRV--5.8, 5.2; EcoRI--11.5.
Insert contains NaeI, PvuII (3), SacI (3), HindIII (2), MstI, BstEII (2), HpaI (2), and EcoRV sites. Four exons demonstrated.
G to A transition in codon 12 leads to activation of this p21.
References

Sukumar S, et al. Induction of mammary carcinomas in rats by nitroso-methylurea involves malignant activation of H-ras-1 locus by single point mutations. Nature 306: 658-661, 1983. PubMed: 6318112

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