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pAMS
pAMS
規(guī)格:
價(jià)格:
編號(hào):B235785
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pAMS
商品貨號(hào)
B235785
Designations
pAMS
GenBank Number

AF010170

培養(yǎng)基
Species
Murine leukemia virus
Depositors
CA Wilson, D Miller
Applications
AM-MLV (ATCC VR-1448) is an engineered broad host range helper virus.
This AM-MLV clone can be used as a reference standard for detection of murine retroviruses.
Vector
Construct size (kb): 11.32800006866455
Insert
DNA: genomic
Insert lengths(kb): 9.03600025177002
Gene product: [AM-MLV*]
Insert Size (kb)
9.036
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Shipped: ?Frozen glycerol stock of ?E. coli containing the plasmid

Comments
Restriction digests of the clone give the following sizes (kb): XhoI--6.8, 4,8; SalI--12.0; BamHI--7.8, 3.6.
The plasmid contains the following restriction sites (approximate kb from nt 1 of the GenBank record): BamHI--3.82, 4.13, 7.55; BglI--4.51, 5.98, 7.30, 10.46; ClaI--5.57, 8.23; EcoRI--0.00, 6.96; HindIII--5.49, 5.65, 7.45; KpnI--0.63, 3.45, 8.86;
NdeI--5.99, 9.27; PstI--1.16, 1.33, 10.58; PvuI--1.01, 2.71, 10.71; SacI--0.56, 3.15, 8.79; SalI--4.30; SmaI--0.62, 5.10, 8.85; XbaI--0.44, 5.92, 8.67; XhoI--2.15, 6.92.
AM-MLV (ATCC VR-1448) is an engineered broad host range helper virus. It is a fusion of amphotropic virus 4070A pol-env with Mo-MLV gag region. It can infect a wide range of mouse cell types (including BALB/c) as well as human and chicken cells.
This AM-MLV clone can be used as a reference standard for detection of murine retroviruses.
The Hybrid Amphotropic/Moloney Murine Leukemia Virus, pAMS, was constructed by inserting the 1541bp SalI-ClaI 4070A pol-env containing fragment into homologous region of Mo-MLV using the cloning vector pBR322 as a backbone.
Classification
Retroviridae, Gammaretrovirus, Mammalian virus group
References

Miller AD, et al. Generation of helper-free amphotropic retroviurses that transduce a dominant-acting, methotrexate-resistant dihydrofolate reductase gene. Mol. Cell. Biol. 5: 431-437, 1985. PubMed: 2985952

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