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pHU10 plasmid in <em>E. coli</em>
pHU10 plasmid in <em>E. coli</em>
規(guī)格:
價(jià)格:詢(xún)價(jià)
編號(hào):B235913
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng)
pHU10 plasmid in E. coli
商品貨號(hào)
B235913
Designations
pHU10 plasmid in E. coli
Depositors
TP Dryja
培養(yǎng)基
Biosafety Level
1
Host
Escherichia coli K12
Vector Information
Total size of construct (kb): 7.2
Marker: ampR
Enzyme to excise insert: HindIII

Vector: pBR322
Vector type: plasmid
Vector size (kb): 4.363
Markers: ampR
Vector ends: HindIII
Comments
Restriction digests of the clone give the following sizes (kb): HindIII--4.4, 2.8; PstI--7.2.
A multiple site polymorphism also detected with XmnI and EcoRI, with a common band of 6.0 and alleles of 8.5 and 10 kb.
An unpublished RFLP is detected by this probe when genomic DNA is digested with EcoRI. The published RFLP is revealed with XmnI.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37°C
References

Dryja TP, et al. Chromosome 13 restriction fragment length polymorphisms. Hum. Genet. 65: 320-324, 1984. PubMed: 6319270

Dryja TP, Morton CC. Mapping of seven polymorphic loci on human chromosome 13 by in situ hybridization. Hum. Genet. 71: 192-195, 1985. PubMed: 4065891

Misra BC, Srivatsan ES. Localization of HeLa cell tumor-suppressor gene to the long arm of chromosome II. Am. J. Hum. Genet. 45: 565-577, 1989. PubMed: 2577469

Lundberg C, et al. Loss of heterozygosity in human ductal breast tumors indicates a recessive mutation on chromosome 13. Proc. Natl. Acad. Sci. USA 84: 2372-2376, 1987. PubMed: 3031679

Leppert M, et al. A primary genetic map of chromosome 13q. Am. J. Hum. Genet. 39: 425-437, 1986. PubMed: 3464200

Thaddeus P Dryja, personal communication

Shipped
freeze-dried
Shipping Information
Freeze-dried E. coli containing the plasmid
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