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pAS1-CDK2
pAS1-CDK2
規(guī)格:
價(jià)格:
編號(hào):B236044
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pAS1-CDK2
商品貨號(hào)
B236044
Designations
pAS1-CDK2
Species
Homo sapiens, human
培養(yǎng)基
Depositors
SJ Elledge
Applications
in another host, produces protein cyclin-dependent kinase 2
Vector
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pAS1
Intact vector size: 7.100
Type of vector: phagemid
Vector end: XhoI
Vector end: XhoI
Cloning sites: NdeI NcoI SfiI SmaI BamHI SalI
Polylinker sites: NdeI NcoI SfiI SmaI BamHI SalI
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
promoter: ADC1 (ADH), ->
other: GAL4 DNA-binding domain, ->
epitope tag: hemagglutinin (HA), ->
restriction site: EcoRI, ->, 4315
MCS: NdeI...SalI, ->, 4395-4422
terminator: ADC1
replicon: pMB1
marker(s): ampR
replicon: 2 micron
marker(s): TRP1, ->
replicon: f1
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CDK2
Gene name: cyclin-dependent kinase 2
Contains complete coding sequence?: U
Localization: 12 q13
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb):
Cross references:
Gene product: cyclin-dependent kinase 2 [CDK2]
Target Gene: cyclin-dependent kinase 2
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): XhoI--7.2, 0.9; EcoRI--8.2; BamHI--8.2; HindIII--4.6, 2.0, 0.9, 0.7; BamHI/HindIII--4.6, 1.8, 0.9, 0.7, 0.2.
Used as a negative control in the 2-hybrid cloning system.
References

Elledge SJ, et al. CDK2 encodes a 33-kDa cyclin A-associated protein kinase and is expressed before CDC2 in the cell cycle. Proc. Natl. Acad. Sci. USA 89: 2907-2911, 1992. PubMed: 1372993

Harper JW, et al. The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases. Cell 75: 805-816, 1993. PubMed: 8242751

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