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PKCepsilon(E3)
PKCepsilon(E3)
規格:
價格:
編號:B236245
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
PKCepsilon(E3)
商品貨號
B236245
Designations
PKCepsilon(E3)
GenBank Number

X65293

培養基
Species
Homo sapiens, human
Depositors
DJ Burns
Applications
in another host, produces protein protein kinase C, epsilon
Vector
Construct size (kb): 16.20000076293945
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBlueBac
Intact vector size: 14.000
Type of vector: phagemid
Vector end: NheI
Vector end: NheI
Cloning sites: NheI
Polylinker sites:
Host range: insect cells; Escherichia coli
Features (with orientation and position when available):
insert detection: lac
marker(s): ampR
promoter: polyhedrin
replicon: pMB1, f1, SV40
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: PRKCE
Gene name: protein kinase C, epsilon
Contains complete coding sequence?: Y
Tissue: brain
Type of DNA: cDNA
Insert end: NheI
Insert end: NheI
Insert size (kb): 2.2
Cross references: DNA Seq. Acc.: X65293
Insert lengths(kb): 2.200000047683716
Tissue: brain
Gene product: protein kinase C, epsilon [PRKCE]
Target Gene: protein kinase C, epsilon
Insert Size (kb)
2.200
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): NheI--14.0, 2.3; HindIII--9.5, 4.4, 1.1, 1.0, 0.4; PstI--14.0, 1.3, 0.8, 0.2; PvuII--3.5, 3.1, 2.5, 2.4, 1.6, 1.4, 1.25, 0.4; SmaI--14.0, 2.1.
The insert contains the following restriction sites (approximate kb from the 5' end): HindIII--1.92; PstI--0.32, 1.54, 1.74; PvuII--1.16.
Constructed from the 5'NcoI/3'EcoRI fragment (nt 14-2185 of the sequence record) using NheI adaptors.
References

Basta P, et al. Sequence and expression of human protein kinase C-epsilon. Biochim. Biophys. Acta 1132: 154-160, 1992. PubMed: 1382605

David J Burns, personal communication

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