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HIBBS11

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編號:B236490

品牌:Mingzhoubio

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DNA

RNA

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產品名稱

HIBBS11

商品貨號

B236490

Designations

HIBBS11

GenBank Number

T09304

培養基

Species

Homo sapiens, human

Vector

Construct size (kb): 4.599999904632568

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lafmid BA
Intact vector size: 3.153
Type of vector: phagemid
Vector end: HindIII
Vector end: NotI
Cloning sites: HindIII, BamHI, NotI, EcoRI
Polylinker sites: HindIII, BamHI, NotI, EcoRI
Other unique sites: ClaI, XmnI, ScaI, ApaLI
Construction: pGEM
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, <-, 100-556
insert detection: lacZ', <-, 594-1119
primer site: M13 universal, ->
MCS: EcoRI/NotI/BamHI/HindIII, ->, 1061-1098
primer site: M13 reverse, <-
promoter: lac, <-, 1164-1192
replicon: pMB1, <-, 1518-1518
marker(s): ampR, <-, 2276-3136
Cross references:

Insert

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CHP*
Gene name: chaperonin HumAP (Cct, TCP1-related)
Contains complete coding sequence?: N
Sequence homology: Homo sapiens t complex-related protein-1, related sequence
3 (matricin)
Tissue: infant brain, 3 months old (from Bento Soares)
Type of DNA: cDNA
Insert 5' end: HindIII
Insert 3' end: NotI
Insert size (kb): 1.3
Cross references: DNA Seq. Acc.: L20509
Nucleotides 1-364 of the insert correspond to
nucleotides 709-1072 of L20509 with 90% identity.
DNA Seq. Acc.: T09304; EST: EST07197; Primer: M13 Reverse;
THC id: THC123995
DNA Seq. Acc.: X74801
Nucleotides 1-364 of the insert correspond to
nucleotides 626-989 of X74801 with 98% identity.

Insert lengths(kb): 1.299999952316284

Tissue: infant brain, 3 months old (from Bento Soares)

Gene product: chaperonin HumAP (Cct, TCP1-related) [CHP*]

Insert Size (kb)

1.300

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)

Comments

Restriction digests of the clone give the following sizes (kb): HindIII/NotI--3.3, 1.3; EcoRI--3.6, 1.1.

Based on the size of the insert and the alignment with GenBank X74801 (1901 nt, CDS 1-1635), this clone appears to contain part of the coding region.

The cDNAs (>95%) are cloned directionally with the 5' end adjacent to the M13 reverse primer and HindIII.

References

Adams MD, et al. Rapid cDNA sequencing (expressed sequence tags) from a directionally cloned human infant brain cDNA library. Nat. Genet. 4: 373-380, 1993. PubMed: 8401585

Mark D Adams, personal communication

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