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NU-DUL-1
NU-DUL-1
規(guī)格:
價(jià)格:
編號(hào):B236760
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
NU-DUL-1
商品貨號(hào)
B236760
Organism
Homo sapiens, human
Tissue
peritonial effusion; derived from metastatic site: malignant cerebrospinal fluid

培養(yǎng)基
Cell Type
lymphocyte
Product Format
frozen
Morphology
early B-cell
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
undifferentiated lymphoma, non-Burkitt's type
Age
43 years
Gender
male
Ethnicity
Caucasian
Applications
The Nu-DUL-1 cell line is a valuable resource for studying the biological behavior of undifferentiated lymphoma cells.
The Nu-DUL-1 cell line will aid molecular and cellular studies designed to identify the biological significance of genomic rearrangements.
Storage Conditions
liquid nitrogen vapor phase
Karyotype
44, X (hypodiploid), +der(4)t(4;?)(q31;?). -6, -17, -18, -21, +2mar,der(1)t(1;?)(q32;?), del(4)(q25q31), del(5)(p12), del(6)(q21), del(8)(q24), der(11)t(11;?)(q21;?), der(12)t(12;?)(p13;?), der(14)t(14;?)(q13;), ter rea(19;21)(q13;q22)
Images
CRL-2969 Micrograph
Clinical Data
43 years
Caucasian
Male
Antigen Expression
LN-1+
LN-2+
LN-3+(anti-HLA-Dr)
BA-1+ (CD24)
CALLA (J5)(common ALL antigen; CD10)+
BA-2 - (CD9)
OKM1 - (myeloid marker)
Leu-7 - (natural killer T cell marker)
OKT-3 -, OKT-4 -, OKT-6 -, OKT-8 -, OKT-11 - (T cell markers)
BM-1 -, BM-2 -, BM-3 - ,BM-4 -, BM-5 - (myeloid markers)

EBNA - (Epstein-Barr Nuclear Antigen)
Genes Expressed
sIg -
Comments
The depositor states that this cell line is negative for the Epstein-Barr Virus (EBV).
ATCC confirmed this cell line is negative for the presence of Epstein-Barr viral DNA sequences via PCR.

The NU-DUL-1 cell line represents a very unusual undifferentiated lymphoma.
The NU-DUL-1 cell line has a chromosome 14 translocation, but not from chromosome 11 or 18 and may represent a new variant.
This cell line is hypodiploid with the presumed loss of a Y chromosome.
NU-DUL-1 cells express markers of early B-cell differentiation.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Cultures can be maintained by the addition of fresh medium. An inoculum of 2.0 x 105to 3.0 x 105cells/mL is recommended. Subculture when the cell concentration is between 8.0 x 105 and 1.0 x 106 cells/mL.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:5 is recommended.
Medium renewal: every 2 to 3 days
Cryopreservation
Freeze medium: complete growth medium supplemented with an additional 20% fetal bovine serum and 10% DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile

D5S818: 12, 14
D13S317: 8, 13
D7S820: 11, 13
D16S539: 11, 13
vWA: 16, 17
THO1: 9, 9.3
Amelogenin: X
TPOX: 8
CSF1PO: 11, 12

Name of Depositor
A Epstein
Year of Origin
1985
References

Epstein AL, Kaplan HS. Feeder layer and nutritional requirements for the establishment and cloning of human malignant lymphoma cell lines. Cancer Res. 39(5):1748-1759, 1979. PubMed: 371794

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