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pmPit1

規格:

價格:

編號:B236831

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pmPit1

商品貨號

B236831

Designations

pmPit1

Species

Mus musculus, mouse

培養基

Depositors

SA Camper

Vector

Construct size (kb): 3.630000114440918

Insert

DNA: cDNA

Insert lengths(kb): 0.671999990940094

Tissue: pituitary

Gene product: pituitary specific transcription factor-1 [Pit1]

Insert Size (kb)

0.672

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

The insert contains RsaI sites 479 nt and 642 nt from the BamHI cloning site.

Restriction digests of the clone give the following sizes (kb): BamHI/HindIII--3.0, 0.67; EcoRI--3.6; HindIII--3.6; BamHI--3.6; PstI--uncut.

Mapped to chromosome 16 by linkage with dw (2.7 cM at 95% confidence level).

The orientation in the vector is such that aa 47 (the 5' end) is at the BamHI site and aa 269 (the 3' end) is at the HindIII site.

Cloned by PCR amplification from cDNA using primers with HindIII and BamHI linkers. The product corresponds to aa 47 to aa 269.

Classification

Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod

References

Camper SA, et al. The Pit-1 transcription factor gene is a candidate for the murine snell dwarf mutation. Genomics 8: 586-590, 1990. PubMed: 1981057

Sally A Camper, personal communication

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