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pNKY150 [Tn10 URA Blaster]
pNKY150 [Tn10 URA Blaster]
規格:
價格:
編號:B236847
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pNKY150 [Tn10 URA Blaster]
商品貨號
B236847
Designations
pNKY150 [Tn10 URA Blaster]
Species
Saccharomyces cerevisiae Meyen ex E.C. Hansen
培養基
Depositors
N Kleckner
Applications
A plasmid containing an excisable URA3 cartridge for gene disruption that permits repeated use of URA3 selection in constructing multiply disrupted yeast strains.
Vector
Construct size (kb): 8.5
Insert
DNA: genomic
Insert lengths(kb): 4.599999904632568
Gene product: uridine monophosphate synthetase( UMP synthase, orotate phosphoribosyltransferase, orotidine 5'-phosphate decarboxylase, orotate phosphoribosyltransferase 1) [URA3]
Insert Size (kb)
4.600
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): BamHI/BglII--5.0, 4.2; BamHI--9.2; EcoRI--9.2; PstI--6.6, 2.6; XbaI--uncut.
Used by inserting the excised cassette into a cloned target gene, isolating a restriction fragment containing the cassette with flanking target gene sequences, transforming Ura3- strains, and selecting for stable integrants.
Loss of the cassette (and one of the flanking IS10 repeats) by recombination can be selected by 5-fluoro-orotic acid resistance.
A plasmid containing an excisable URA3 cartridge for gene disruption that permits repeated use of URA3 selection in constructing multiply disrupted yeast strains.
The BglII/BamHI cassette contains URA3 flanked by 1.5 kb direct repeats from insertion sequence IS10.
Classification
Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
References

Alani E, et al. A method for gene disruption that allows repeated use of URA3 selection in the construction of multiply disrupted yeast strains. Genetics 116: 541-545, 1987. PubMed: 3305158

Riles L, et al. Physical maps of the six smallest chromosomes of Saccharomyces cerevisiae at a resolution of 2.6-kilobase pairs. Genetics 134: 81-150, 1993. PubMed: 8514151

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