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pTUM1.4
pTUM1.4
規(guī)格:
價格:
編號:B236994
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pTUM1.4
商品貨號
B236994
Designations
pTUM1.4
Species
Turnip mosaic virus
培養(yǎng)基
Depositors
J Laliberte
Vector
Construct size (kb): 4.400000095367432
Insert
DNA: cDNA
Insert lengths(kb): 1.447000026702881
Gene product: capsid, 33 kDa
Insert Size (kb)
1.447
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.95, 0.9, 0.6; PstI--4.4; BamHI/HindIII--2.95, 1.45; XhoI--4.4; XbaI--3.0, 1.4.
The insert contains the following restriction sites, in kb from the 5' end: ClaI--0.156; EcoRI--0.906.
Isolated from naturally infected Brassica napus ssp. napobrassica (rutabaga) in the region of L'Assomption, Quebec, Canada.
Contains the complete capsid protein gene, part of the NIb gene, and part of the 3' end.
The 5' end of the insert begins 204 bp 5' to the published sequence and end at nt -506 of the published sequence.
Transcription from the T7 promoter gives the plus strand.
Classification
Potyvirus
References

Tremblay MF, et al. Sequence of the 3' terminal region of turnip mosaic virus RNA and the capsid protein gene. J. Gen. Virol. 71: 2769-2772, 1990. PubMed: 2254757

Jean-Francois Laliberte, personal communication

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