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CBRM1/23
CBRM1/23
規(guī)格:
價(jià)格:
編號(hào):B237047
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
CBRM1/23
商品貨號(hào)
B237047
Organism
Mus musculus, mouse
Tissue
spleen
培養(yǎng)基
Cell Type
hybridoma:lymphoblast B lymphocyte; somatic cell hybri
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain
BALB/c
Applications
[Pubmed: 7679388] The monoclonal antibodies produced by these hybridomas will be new insights into how different domains in the integrin alpha and beta subunits may interact.
Storage Conditions
liquid nitrogen vapor phase
Images
Derivation
Mice (BALB/c) were immunized with purified Mac1 (CD11b/CD18). Spleen cells were fused with P3X63Ag8.653 (ATCC CRL-1580). Monoclonal antibodies from selected hybridomas recognized the Mac-1 alpha subunits on the surface of neutrophils. [Pubmed: 7679388] The monoclonal antibodies produced by these hybridomas will be new insights into how different domains in the integrin alpha and beta subunits may interact. [Pubmed: 9614126]
Genes Expressed
immunoglobulin; monoclonal antibody against C-terminal epitope of human Mac-1
Cellular Products
immunoglobulin; monoclonal antibody against C-terminal epitope of human Mac-1
Comments
Mice (BALB/c) were immunized with purified Mac1 (CD11b/CD18). Spleen cells were fused with P3X63Ag8.653 (ATCC CRL-1580). Monoclonal antibodies from selected hybridomas recognized the Mac-1 alpha subunits on the surface of neutrophils. [Pubmed: 7679388] The monoclonal antibodies produced by these hybridomas will be new insights into how different domains in the integrin alpha and beta subunits may interact. [Pubmed: 9614126]
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing
Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(4) to 1 X 10(5) viable cells/ ml. Maintain cultures at a cell concentration between 5 X 10(5) and 1 X 10(6) cells/ml.
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype
IgG2a; Kappa light chain
Name of Depositor
TA Springer
Year of Origin
November 27, 1991
References

Lu C, et al. The structure of the beta-propeller domain and C-terminal region of the integrin alphaM subunit. Dependence on beta subunit association and prediction of domains.. J. Biol. Chem. 273: 15138-15147, 1998. PubMed: 9614126

Diamond MS, et al. The I domain is a major recognition site on the leukocyte integrin Mac-1 (CD11b/CD18) for four distinct adhesion ligands. J. Cell Biol. 120: 1031-1043, 1993. PubMed: 7679388

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