黄色小说视频-日本少妇高潮抽搐-国内自拍av-天堂中文资源在线-蜜桃视频在线入口www-91久久综合-亚洲视频在线一区-日日夜夜拍-国产精品资源在线观看-欧美激情国产精品免费-人妻少妇久久中文字幕-成人免费无码大片a毛片-香蕉污视频在线观看-日本电影成人-国内精品久久99人妻无码

歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > pBSL130 B237119
pBSL130
pBSL130
規格:
價格:
編號:B237119
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pBSL130
商品貨號
B237119
Designations
pBSL130
Depositors
MF Alexeyev
培養基
Biosafety Level
1
Host
Distribution host: Escherichia coli DH5
Vector Information
Size (kb): 5.0349998474121090
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: sBSL98
Intact vector size: 3.100
Type of vector: phagemid
Vector end: mLuI
Vector end: mLuI
Cloning sites:
Polylinker sites: KpnI ApaI XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI
SpeI XbaI NotI/EagI BstXI SacII SacI mLuI
Construction: pBluescript
Host range: Escherichia coli
Features (with orientation and position when available):
MCS: KpnI...SacI mLuI, ->
MCS: mLuI KpnI...SacI, ->
replicon: pMB1
marker(s): ampR, <-
replicon: f1, <-
Cross references:
Vector: pBSL130 (phagemid)
Promoters: Promoter for in vitro transcription T7 (phi10)
Construction: pBluescript
Marker(s):ampR,spcR,strR
Construct size (kb): 5.034999847412109
Features: marker(s): ampR
promoter for in vitro transcription: T7 (phi10)
replicon: f1
replicon: pMB1
MCS: KpnI...SacI MluI
MCS: MluI KpnI...SacI
coding sequence: aadA (spcR, strR)
Applications
contains easily purifiable cassette(s) for construction omega-aadA1
Comments
Restriction digests of the clone give the following sizes (kb): MluI--3.0, 2.0; EcoRI--2.8, 2.1; BamHI--2.8, 2.1.
Plasmid containing an easily purified spcR (strR) cassette (flanked by direct repeats of the a multiple cloning sequence) for use in construction.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Alexeyev MF, et al. Improved antibiotic-resistance gene cassettes and omega elements for Escherichia coli vector contruction and in vitro deletion/insertion mutagenesis. Gene 160: 63-67, 1995. PubMed: 7628718

Shipped
freeze-dried
Shipping Information
Distributed: freeze-dried
  • 王經理
    手機號:17280875617
    QQ:1438578920
  • 胡經理
    手機號:13345964880
    QQ:2438244627
  • 周經理
    手機號:17757487661
    QQ:1296385441
  • 于經理
    手機號:18067160830
    QQ:2088210172
  • 沈經理
    手機號:19548299266
    QQ:2662369050
  • 李經理
    手機號:13626845108
    QQ:972239479