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pYNH15
pYNH15
規格:
價格:
編號:B237208
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pYNH15
商品貨號
B237208
Designations
pYNH15
Species
Homo sapiens, human
培養基
Applications
Also detects a 2-allele RsaI RFLP (3.0, 1.7 kb) not tested in pedigree studies.
Vector
Construct size (kb): 6.0
Insert
DNA: genomic
Insert lengths(kb): 3.200000047683716
Gene product: DNA Segment, single copy [D12S17]
Alleles: A1, A2, A3
Insert Size (kb)
3.200
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.8, 2.2, 1.0.
An EcoRI/HindIII double digest shows insert fragment sizes of 2.2 and 1.0 kb.
The MspI RFLP shows significant linkage to autosomal recessive vitamin D dependency type I (thetam = 0.018, Z = 3.2).
D12S17 and D12S25 detect the same polymorphism and therefore represent the same locus.
Codominant segregation of the MspI polymorphism has been demonstrated in 42 3-generation families. Also detects a 2-allele RsaI RFLP (3.0, 1.7 kb) not tested in pedigree studies.
Enzyme(s) not detecting polymorphism: BamHI, BglII, EcoRI, HindIII, PstI, PvuII, TaqI.
References

Labuda M, et al. Mapping autosomal recessive vitamin D deficiency type I to chromosome 12q14 by linkage analysis. Am. J. Hum. Genet. 47: 28-36, 1990. PubMed: 1971995

Hughes AE. D12S17 and D12S25 identify the same MspI RFLP. Nucleic Acids Res. 19: 1724, 1991. PubMed: 1709282

Nakamura Y, et al. Isolation and mapping of a polymorphic DNA sequence pYNH15 on chromosome 12q [D12S17]. Nucleic Acids Res. 16: 779, 1988. PubMed: 2893347

Yusuke Nakamura, personal communication

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