Intact vector size: 6.143
Type of vector: phagemid
Cloning sites: ClaI NotI ApI
Polylinker sites: BamHI EcoRI SmaI SalI XhoI NotI ApaI
Other unique sites: BglII BssHI EcoRV PstI PvuI ScaI SpeI
Construction: pUC19, pGPi-2, pIC-20H
Host range: mammalian cells; Escherichia coli
Features (with orientation and position when available):
Promoter: CMV, ->, 1-686
Promoter: lac, ->, 687-847
Leader Peptide: beta-gal leader sequence, 848-886
Coding Sequence: human GST pi, ->, 887-1516
Other: thrombin cleavage site, 1517-1524
MCS: BamHI...ApaI, 1525-1566
Stop Codons: TGA, 1570-1572
Replicon: f1 ori, 1895-2413
Promoter for Expression: SV40, ->, 2466-2800
Replicon: SV40 ori, 2669-2754
Coding Sequence: Neo, ->, 2836-3630
Terminator: SV40 polyadenylation, 3804-3939
Replicon: pMB1, 4373-4373
Marker(s): ampR, <-, 5131-5991
The pGFLEX expression system allows production of target proteins fused to either the N or C terminus of the GST pi protein and provides rapid purification of target proteins as either GST fusions or native proteins after cleavage with thrombin.
Manoharan HT, et al. High-level production and purification of biologically active proteins from bacterial and mammanlian cells using the tandem pGFLEX expression system. Gene 193: 229-237, 1997. PubMed: 9256081
Herbert Manoharan, personal communication
