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p63
p63
規格:
價格:
編號:B237361
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
p63
商品貨號
B237361
Designations
p63
GenBank Number

X06538

培養基
Species
Homo sapiens, human
Vector
Construct size (kb): 4.539999961853027
Insert
DNA: cDNA
Insert lengths(kb): 1.639999985694885
Tissue: breast adenocarcinoma MCF7 cell line
Gene product: retinoic acid receptor, alpha [RARA]
Alleles: A1, A2
Insert Size (kb)
1.640
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Insert contains the following sites separated by (bp): KpnI- 380- PstI- 125- SacI- 495- PstI- 155- SmaI- 285- SmaI- 200- EcoRI.
Restriction digests of the clone give the following sizes (kb): EcoRI--5.0; KpnI--5.0; EcoRI/KpnI--3.1, 1.8; SacI--5.0; PstI--3.7, 0.7, 0.5.
Contains a 1296 nt open reading frame (starting at position 317) that encodes a 432 aa protein. RNA can be transcribed in vitro from vector T7 promoter.
This appears to be a retinoic acid-inducible, trans-acting enhancer factor.
Enzyme(s) not detecting polymorphism: ApaI, ApaLI, BamHI, BanI, BanII, BglII, BstNI, BxtXI, DraI, EcoO109, EcoRI, HindIII, MspI, NheI, PvuII, ScaI, StuI, TaqI, Tth111I, XmnI (in a minimum of 10 independent chromosomes).
References

Mattei MG, et al. Mapping of the human retinoic acid receptor to the q21 band of chromosome 17. Hum. Genet. 80: 186-188, 1988. PubMed: 2844649

Zelent A, et al. Cloning of murine alpha and beta retinoic acid receptors and a novel receptor gamma predominantly expressed in skin. Nature 339: 714-717, 1989. PubMed: 2544807

Arveiler B, et al. A PstI RFLP for the human retinoic acid receptor in 17q21. Nucleic Acids Res. 16: 6252, 1988. PubMed: 2899875

Petkovich M, et al. A human retinoic acid receptor which belongs to the family of nuclear receptors. Nature 330: 444-450, 1987. PubMed: 2825025

Pierre Chambon, personal communication

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