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pBSL15

規格:

價格:

編號:B237471

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pBSL15

商品貨號

B237471

Designations

pBSL15

Depositors

MF Alexeyev

培養基

Biosafety Level

Vector Information

Size (kb): 3.9000000953674320

Vector: pBSL15 (plasmid)

Construction: pUC18, pK18

Marker(s):ampR,kanR

Construct size (kb): 3.900000095367432

Features: marker(s): ampR
marker(s): kanR
replicon: pMB1
MCS: HindIII...EcoRI
MCS: NheI...HindIII

Applications

contains easily purifiable cassette(s) for construction aminoglycoside phosphotransferase kanamycin resistance, neomycin resistance

vector permitting positive selection for inserts

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI--2.8, 1.2; BamHI--2.75, 1.3; HindIII--2.7, 1.4.

The nptII gene was modified by site-directed mutagenesis to remove PstI and SphI sites.

An inverted repeat of the 271 bp NdeI/EcoRI fragment of pUC18 surrounds the kanR cassette. If this vector is ligated without a stuffer fragment, this inverted repeat prevents replication, thus providing positive selection for plasmids with inserts.

Plasmid with an easily purified kanR cassette (flanked by inverted repeats of the pUC18 multiple cloning sequence) for construction. pBSL15 and pBSL86 differ in the orientation of the repeats.

Media

LB plus ampicillin (50 mcg/ml) plus kanamycin (50 mcg/ml)

Growth Conditions

Temperature: 37.0°C

References

Alexeyev MF. Three kanamycin resistance gene cassettes with different polylinkers. BioTechniques 18: 52-55, 1995. PubMed: 7702853

Shipped

freeze-dried

B196725：pBSL159

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