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pHAL [p3964Full]
pHAL [p3964Full]
規格:
貨期:
編號:B237483
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pHAL [p3964Full]
商品貨號
B237483
Designations
pHAL [p3964Full]
GenBank Number

J05653

Species
Rattus norvegicus, rat
Depositors
RR McInnes
Vector
Construct size (kb): 5.138000011444092
Insert
DNA: cDNA
Insert lengths(kb): 2.178999900817871
Tissue: liver
Gene product: histidine ammonia-lyase(histidase) [HAL]
Insert Size (kb)
2.179
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.9, 0.24; XhoI--3.9, 0.96, 0.24; SmaI--4.7, 0.48; PvuII--2.5 (doublet).
The insert contains the following restriction sites (approximate kb from the 5' end): KpnI--0.05; SmaI--0.45; EcoRV--1.0; XhoI--1.02, 1.98; NcoI--1.6; EcoRI--1.95.
Contains the complete coding sequence, with 8 nt of the 5' untranslated region and the complete 3' untranslated region.
The 1.95 kb EcoRI fragment detects RNA of 2.5 kb in rat liver and rat skin (major) and 7.3 and 4.4 (minor).
The 5' end of the insert is adjacent to the T7 promoter of the vector.
References

Taylor RG, et al. Cloning and expression of rat histidase. Homology to two bacterial histidases and four phenylalanine ammonia-lyases. J. Biol. Chem. 265: 18192-18199, 1990. PubMed: 2120224

Taylor RG, et al. Localization of histidase to human chromosome region 12q22----q24.1 and mouse chromosome region 10C2----D1. Cytogenet. Cell Genet. 56: 178-181, 1991. PubMed: 2055114

Roderick R McInnes, personal communication

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