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c8083
c8083
規格:
價格:
編號:B237857
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
c8083
商品貨號
B237857
Designations
c8083
Species
Saccharomyces cerevisiae Meyen ex E.C. Hansen
培養基
Depositors
L Riles, MV Olson
Vector
Construct size (kb): 50.0
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pHC79
Intact vector size: 6.430
Type of vector: cosmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: EcoRI ClaI BamHI SalI EcaI PstI
Polylinker sites:
Construction: pBR322, Charon 4A
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, tetR
replicon: pMB1
terminator: none
Cross references: DNA Seq. Acc.: L08873
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Saccharomyces cerevisiae
Gene symbol: TOP3
Gene name: topoisomerase (DNA) III
Contains complete coding sequence?: Y
Chromosome: XII
Genome: Saccharomyces cerevisiae
Gene symbol: EST1
Gene name: telomere elongation protein (probable telomerase)
Contains complete coding sequence?: Y
Chromosome: XII
Genome: Saccharomyces cerevisiae
Gene symbol: CDC42
Gene name: cell division cycle 42 (GTP-binding protein, 25kD)
Contains complete coding sequence?: Y
Chromosome: XII
Type of DNA: genomic
Insert end: MboI
Insert end: MboI
Insert size (kb): 43.5
Cross references:
Insert lengths(kb): 43.5
Gene product: cell division cycle 42 (GTP-binding protein, 25kD) [EST1]
Insert Size (kb)
43.5
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: frozen, uncharacterized
Comments
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
Classification
Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
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