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pG143/T2
pG143/T2
規(guī)格:
價格:
編號:B237889
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pG143/T2
商品貨號
B237889
Designations
pG143/T2
Species
Saccharomyces cerevisiae Meyen ex E.C. Hansen
培養(yǎng)基
Depositors
A Tzagoloff
Vector
Construct size (kb): 17.5
Insert
DNA: genomic
Insert lengths(kb): 9.300000190734863
Gene product: transcription activation factor HAP3 [HAP3]
Insert Size (kb)
9.300
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
The insert contains the following sites separated by (approximate kb): EcoRI- 2.2- XbaI- 0.6- SstI- 0.7- EcoRI- 0.9- BamHI- 0.25- SphI- 0.55- SstI- 0.1- SphI- 0.7- XbaI.
Restriction digests of the clone give the following sizes (kb): 12.0, 4.65, 1.0; BamHI--17.5; SmaI/SalI--7.6 (doublet), 2.25; KpnI--uncut.
The HAP3 gene is included in a 1.6 kb BamHI/XbaI fragment.
The HAP3 locus encodes 2 overlapping transcripts of 0.57 kb and 4.0 kb read from opposite strands. The smaller mRNA, encoding a protein of 144 aa, directs the synthesis of a protein required for UAS2 activity.
Classification
Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
References

Hahn S, et al. The HAP3 regulatory locus of Saccharomyces cerevisiae encodes divergent overlapping transcripts. Mol. Cell. Biol. 8: 655-663, 1988. PubMed: 2832732

Alexander Tzagoloff, personal communication

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