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pCLL4
pCLL4
規格:
價格:
編號:B237921
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pCLL4
商品貨號
B237921
Designations
pCLL4
Depositors
American Cyanamid Co., JS Feitelson, American Cyanamid Co.
培養基
U.S. Patent
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Biosafety Level
1
Vector Information
Size (kb): 10.36
Vector: pCLL4 (plasmid)
Construction: pAkij1, pIJ39
Marker(s):ampR,tsrR
Construct size (kb): 10.36
Features: marker(s): ampR
marker(s): tsrR
replicon: pAkij1
Applications
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): HindIII--9.0, 2.1; PstI--10.5; KpnI--10.5.
Cloning into the PstI site inactivates the ampicillin marker used for selection in Escherichia coli.
The pAkij1 ori may be useful in other taxa such as Bacillus, Staphylococcus and other Actinomycetes, including Streptosporangium, Actinoplanes, Nocardia, and Micromonospora.
Recommendation for verification: HindIII--8.37, 1.99; PstI--10.36; KpnI--9.47, 0.89
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Feitelson JS. Multifunctional plasmid vectors from Actinomadura and Escherichia coli. US Patent 5,002,891 dated Mar 26 1991

Feitelson JS, et al. Genetic and biochemical characterization of the red gene cluster of streptomyces coelicolor A3(2). J. Gen. Microbiol. 131: 2431-2441, 1985. PubMed: 2999302

Shipped
freeze-dried
Shipping Information

Shipped: ?Freeze dried E. coli containing the plasmid

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