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pMAP3
pMAP3
規(guī)格:
價格:
編號:B237925
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pMAP3
商品貨號
B237925
Designations
pMAP3
GenBank Number

D00117

培養(yǎng)基
Species
Bacillus thuringiensis subsp. kurstaki de Barjac and Lemille
Depositors
Monsanto Co., FJ Perlak, Monsanto Co.
Applications
produces protein crystal protein (delta endotoxin)
Vector
Construct size (kb): 10.80000019073486
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pUC8
Intact vector size: 2.700
Type of vector: plasmid
Vector end: BamHI
Vector end: PstI
Cloning sites: EcoRI SmaI XmaI BamHI SalI AccI HincII PstI HindIII
Polylinker sites: EcoRI SmaI XmaI BamHI SalI AccI HincII PstI HindIII
Construction: pBR322, Escherichia coli lacZalpha, M13mp8
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac
replicon: pMB1
terminator: none
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Bacillus thuringiensis subsp. kurstaki
Gene name: crystal protein (delta endotoxin)
Contains complete coding sequence?: Y
Strain: HD-1
Type of DNA: plasmid
Insert 5' end: BamHI
Insert 3' end: PstI
Insert size (kb): 8.1
Cross references: DNA Seq. Acc.: D00117
Insert lengths(kb): 8.100000381469727
Gene product: crystal protein (delta endotoxin)
Target Gene: crystal protein (delta endotoxin)
Insert Size (kb)
8.100
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI/PstI--8.1, 2.7; EcoRI--5.2, 1.9 (doublet), 0.7, 0.6; HindIII--8.0, 1.5, 1.1.
The following microorganisms are suitable for recombination and plant-colonization: Pseudomonas, Agrobacterium, Rhizobium, Erwinia, Azotobacter, Azospirillum, Klebsiella, Alcaligenes and Flavobacterium.
The coding sequence within the insert can be integrated into the chromosome of one of several plant-colonizing microorganisms to protect the colonized plant against insect pests.
The complete coding sequence for the toxin is contained within an internal 4.6 kb HpaI-PstI fragment.
References

Obukowicz MG, et al. Combatting plant insect pests with plant-colonizing microorganisms containing the toxin gene B. thuringiensis as a chromosomal insertion. US Patent 5,229,112 dated Jul 20 1993

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
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