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pAL49

規格:

價格:

編號:B238239

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pAL49

商品貨號

B238239

Designations

pAL49

Depositors

AC Leonard

培養基

Biosafety Level

Vector Information

Size (kb): 7.0000000000000000

Vector: pAL49 (plasmid)

Construction: Escherichia coli oriC, pML21, Tn903

Marker(s):kanR,neoR

Construct size (kb): 7.0

Features: marker(s): kanR, neoR
replicon: oriC

Applications

vector for other uses

Comments

The plasmid minichromosome exhibits a moderate degree of instability and should be propagated in recA- strains grown in at least 50 mcg/ml kanamycin.

A plasmid which replicates from the resident chromosome origin of replication, oriC.

Media

ATCC^® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin

Growth Conditions

Temperature: 37.0°C

References

Leonard AC, Helmstetter CE. Cell cycle-specific replication of Escherichia coli minichromosomes. Proc. Natl. Acad. Sci. USA 83: 5101-5105, 1986. PubMed: 3523483

Leonard AC, et al. Involvement of DNA superhelicity of minichromosome maintenance in Escherichia coli. J. Bacteriol. 161: 687-695, 1985. PubMed: 2981821

Shipped

freeze-dried

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