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YEplac112

規(guī)格:

價格:

編號:B238265

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產品名稱

YEplac112

商品貨號

B238265

Designations

YEplac112

Depositors

RD Gietz

培養(yǎng)基

Biosafety Level

Vector Information

Size (kb): 4.989

Vector: YEplac112 (plasmid)

Promoters: Promoter lac

Construction: pUC19

Marker(s):TRP1,ampR

Construct size (kb): 4.989

Features: insert detection: lacZ'
marker(s): TRP1
marker(s): ampR
promoter: lac
replicon: 2 micron ori
replicon: pMB1
MCS: HindIII...EcoRI

Applications

YE-type (episomal) shuttle vector

vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase

Comments

This is a YE-type (yeast episomal) vector with the TRP1 marker.

The HindIII, PstI and the XbaI sites from the TRP1 gene of the S. cerevisiae were removed by using oligo-directed mutagenesis.

One of 9 shuttle vectors (ATCC 87585 - 87593) allowing lacZ detection of cloned inserts and containing the pUC19 MCS with all 10 cloning sites unique. Vectors differ in mode of replication (YE, YC or YI-type) and yeast marker (LEU2, URA3 or TRP1).

Restriction digests of the clone give the following sizes (kb): AvaI--2.9, 1.3, 0.8; HindIII--4.9; PstI--4.9.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

Gietz RD, Sugino A. New yeast Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites. Gene 74: 527-534, 1988. PubMed: 3073106