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HRRM2
HRRM2
規(guī)格:
價(jià)格:
編號(hào):B238376
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng)
HRRM2
商品貨號(hào)
B238376
Designations
HRRM2
GenBank Number

X59618

培養(yǎng)基
Species
Homo sapiens, human
Depositors
Y Yen
Vector
Construct size (kb): 5.125
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pCRII
Intact vector size: 3.900
Type of vector: phagemid
Vector end: TA site
Vector end: TA site
Cloning sites: TA cloning between EcoRI sites
Polylinker sites: NsiI HindIII KpnI SacI BamHI SpeI BstXI EcoRI EcoRI EcoRV
BstXI NotI AvaI XmaIII XhoI NsiI XbaI ApaI
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR, kanR
promoter: lac, SP6, T7
replicon: pMB1
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: RRM2
Gene name: ribonucleotide reductase M2 polypeptide
Contains complete coding sequence?: Y
Chromosome: 2; Localization: 2 p25-p24
Type of DNA: cDNA
Insert 5' end: Modification: BamHI site
Insert 3' end: Modification: HindIII site
Insert size (kb): 1.17
Cross references: DNA Seq. Acc.: X59618
Nucleotides ~1~1169 of the insert correspond to
nucleotides 195-1364 of X59618.
Insert lengths(kb): 1.169999957084656
Gene product: ribonucleotide reductase (ribonucleoside-diphosphate reductase) small subunit( ribonucleotide reductase, small subunit, ribonucleotide reductase M2 polypeptide) [RRM2]
Insert Size (kb)
1.170
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--3.8, 1.2; HindIII--4.9; HindIII/EcoRI--3.9, 1.2; PstI--3.2, 1.1, 0.7; SalI--4.9.
The insert contains the following restriction sites (approximate kb from the 5' end): SalI--0.08; PstI--0.68; EcoRI--1.14.
Constructed by PCR using primers that generated a BamHI site upstream of the initiation codon (5' ATCCGGATCCACTATG 3') and a HindIII site downstream of the termination codon (5' TAAATAAGCTTAAGC 3').
References

Pavloff N, et al. Sequence analysis of the large and small subunits of human ribonucleotide reductase. DNA Seq. 2: 227-234, 1992. PubMed: 1627826

Yun Yen, personal communication

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