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T47D-KBluc
T47D-KBluc
規格:
價格:
編號:B238518
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
T47D-KBluc
商品貨號
B238518
Organism
Homo sapiens, human
Tissue
mammary gland; breast/duct; derived from metastatic site: pleural effusion
培養基
Cell Type
epithelial transfected with reporter plasmid
Product Format
frozen
Morphology
epithelial
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
ductal carcinoma
Age
54 years
Gender
female
Applications
They can be used to screen chemicals for estrogenic or anti-estrogenic activity.
Storage Conditions
liquid nitrogen vapor phase
Clinical Data
female
HeLa Markers
N
Comments

The parent cell line was transfected with pGL2.TATA.Inr.luc.neo which contains three estrogen responsive elements upstream of a luc reporter gene. 

The cells were selected for responsiveness to 17-beta-estradiol. They can be used to screen chemicals for estrogenic or anti-estrogenic activity.

Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: 0.2 Units/ml bovine insulin; fetal bovine serum to a final concentration of 10%.
Subculturing
Volumes used in this protocol are for 75cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Note: Subculture cells before or upon reaching confluence. Do not allow cells to become super-confluent.

  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with either Hanks' Balanced Salt Solution (HBSS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 10 to 15 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 2 x 104 to 4 x 104 viable cells/cm2 is recommended.
  6. Incubate cultures at 37C. We recommend that you maintain cultures at a cell concentration between 3 x 104 and 2 x 105 cells/cm2.

Subcultivation Ratio: 1:2 to 1:3 is recommended
Medium Renewal: every 2 to 3 days
Note: The depositor states that supplementing the growth medium with insulin is optional.
Cryopreservation
Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 11,13
D13S317: 12
D16S539: 10
D5S818: 12
D7S820: 11
THO1: 6
TPOX: 11
vWA: 14
Population Doubling Time
36
Name of Depositor
VS Wilson
Year of Origin
January 4, 2000
References

Wilson VS, et al. Development and characterization of a cell line that stably expresses an estrogen-responsive luciferase reporter for the detection of estrogen receptor agonist and antagonists. Toxicol. Sci. 81: 69-77, 2004. PubMed: 15166400

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