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pCMV-SREBP2-468
pCMV-SREBP2-468
規格:
價格:
編號:B238791
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pCMV-SREBP2-468
商品貨號
B238791
Designations
pCMV-SREBP2-468
GenBank Number

NM_004599

培養基
Species
Homo sapiens, human
Depositors
JD Horton, JL Goldstein
Applications
contains sequence sterol regulatory element binding transcription factor 2 sterol regulatory element-binding protein 2
Insert
DNA: cDNA
Insert lengths(kb): 1.480000019073486
Tissue: HeLa cells
Gene product: sterol regulatory element binding transcription factor 2(sterol regulatory element-binding protein 2) [SREBP2]
Target Gene: sterol regulatory element binding transcription factor 2
Insert Size (kb)
1.480
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Shipped: ?Frozen glycerol stock of E. coli containing the plasmid

Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--6.4; SmaI--6.4; EcoRI/SmaI--4.6, 1.8; PstI--5.6, 0.8; BamHI/PstI--5.6, 0.8.
Contains 75 bp of 5'UTR and sequence for amino acids 1-468 of human SREBR2. It is not full length but encodes the transcriptionally active form of the protein.
Insert cannot be excised.
References

Shimano H, et al. Isoform 1c of sterol regulatory element binding protein is less active than isoform 1a in livers of transgenic mice and in cultured cells. J. Clin. Invest. 99: 846-854, 1997. PubMed: 9062341

Horton JD, et al. Activation of cholesterol synthesis in preference to fatty acid synthesis in liver and adipose tissue of transgenic mice overproducing sterol regulatory element-binding protein-2. J. Clin. Invest. 101: 2331-2339, 1998. PubMed: 9616204

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