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pUR288-126K/183K
pUR288-126K/183K
規格:
貨期:
編號:B239445
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 pUR288-126K/183K
商品貨號 B239445
Designations pUR288-126K/183K
Species Tobacco mosaic virus
Depositors WO Dawson
Applications
produces protein 126K protein
produces protein 183K protein
Vector
Construct size (kb): 5.940000057220459
Insert
DNA: cDNA
Insert lengths(kb): 0.7400000095367432
Gene product: 126K protein
Target Gene: 126K protein, 183K protein
Insert Size (kb) 0.740
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.0; HindIII--6.0; EcoRI--5.2, 0.76; BamHI/XbaI--5.2, 0.74.
This clone expresses an antigen for the 126K and 183K proteins of tobacco mosaic virus.
The internal SmaI-XbaI 126K gene of pTMV204 (nt 259-1000) was ligated to BamHI linkers at the SmaI end and then ligated into BamHI-XbaI digested pUR288. The correct reading frame was confirmed by sequencing.
Classification Tobamovirus
References

Dawson WO, et al. cDNA cloning of the complete genome of tobacco mosaic virus and production of infectious transcripts. Proc. Natl. Acad. Sci. USA 83: 1832-1836, 1986.

Lehto K, et al. Time course of TMV 30K protein accumulation in intact leaves. Virology 174: 290-293, 1990. PubMed: 2294644

William O Dawson, personal communication

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