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28S.3
28S.3
規(guī)格:
價格:
編號:B239502
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
28S.3
商品貨號
B239502
Organism
Ictalurus punctatus, channel catfish
Tissue
peripheral blood
培養(yǎng)基
Cell Type
lymphocyte T lymphoblast
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The cells constitutively produce a supernatant factor(s) with growth promoting activity [PubMed: 9809582].
The 28S.3 cell line, developed in 1996, is an immortal T cell line spontaneously derived from a long term culture of peripheral blood from a normal channel catfish.
Storage Conditions
liquid nitrogen vapor phase
Karyotype
diploid
Derivation
The 28S.3 cell line, developed in 1996, is an immortal T cell line spontaneously derived from a long term culture of peripheral blood from a normal channel catfish. The cells were subsequently cloned by limiting dilution. The cells are maintained in vitro without restimulation, feeder cells, or exogenous factors. The cells are immunoglobulin negative [PubMed: 9809582]. The cells constitutively produce a supernatant factor(s) with growth promoting activity [PubMed: 9809582].
Comments
The 28S.3 cell line, developed in 1996, is an immortal T cell line spontaneously derived from a long term culture of peripheral blood from a normal channel catfish. The cells were subsequently cloned by limiting dilution. The cells are maintained in vitro without restimulation, feeder cells, or exogenous factors. The cells are immunoglobulin negative [PubMed: 9809582]. The cells constitutively produce a supernatant factor(s) with growth promoting activity [PubMed: 9809582].
Complete Growth Medium
A 1:1 mixture of Leibovitz's L-15 medium with 2 mM L-glutamine, and AIM-V Medium adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.05 mM 2-mercaptoethanol, 87.5%; double distilled water, 10%; heat-inactivated Catfish serum, 2.5%

(Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)


Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 10(5) viable cells/ml.
Interval: Maintain cell density between 5 X 10(5) and 5 X 10(6) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 27.0°C
Name of Depositor
NW Miller
Year of Origin
1996
References

Wilson MR, et al. T-cell receptors in channel catfish: structure and expression of TCR alpha and beta genes. Mol. Immunol. 35: 545-557, 1998. PubMed: 9809582

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