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pCR46 [Bacillus subtilis IS75]
pCR46 [Bacillus subtilis IS75]
規(guī)格:
價(jià)格:
編號(hào):B239548
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pCR46 [Bacillus subtilis IS75]
商品貨號(hào)
B239548
Designations
pCR46 [Bacillus subtilis IS75]
Species
Bacillus coagulans Hammer
培養(yǎng)基
Depositors
OmniGene, Inc., JG Pero, OmniGene, Inc.
Applications
produces protein amylase
Vector
Construct size (kb): 5.900000095367432
Insert
DNA: genomic
Insert lengths(kb): 1.929999947547913
Gene product: amylase [amy]
Target Gene: amylase
Insert Size (kb)
1.930
Media
ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): BamHI--4.4, 1.8; EcoRI--6.4; BglII--6.4; HindIII--6.4.
The BamHI fragment has been used to construct high-level expression vectors for production of fusion proteins.
The additional glycine and serine residues at the carboxy terminus of the protein do not appear to affect amylase activity.
The vector was derived from pBD64 by replacing the PvuII site with a HindIII site.
Altered from the wild-type sequence by inserting a second BamHI site between the last codon and the stop codon.
The order of the major features in this plasmid is: HindIII - BamHI/amylase/BamHI - EcoRI - kanR/BglII - cmlR.
References

Sloma A, et al. Expression of heterologous DNA using the Bacillus coagulans amylase gene. US Patent 5,171,673 dated Dec 15 1992

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
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