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pmIL1AcDNA [murine IL-1alpha cDNA]

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貨期:

編號:B239577

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

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產品名稱

pmIL1AcDNA [murine IL-1alpha cDNA]

商品貨號

B239577

Designations

pmIL1AcDNA [murine IL-1alpha cDNA]

GenBank Number

X01450

Species

Mus musculus, mouse

Depositors

U Gubler

Vector

Construct size (kb): 5.0

Insert

DNA: cDNA

Insert lengths(kb): 2.0

Tissue: P388D1 cell line

Gene product: interleukin 1, alpha [Il1a]

Insert Size (kb)

2.0

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

The insert contains the following restriction sites (nt positions according to the published sequence): HpaI--1188; PstI--373; PvuII--789; ScaI--1111; XbaI--1585.

Restriction digests of the clone give the following sizes (kb): HindIII/BamHI--3.0, 2.0; PstI--5.0; PvuII--2.7, 1.5, 0.8; EcoRI--uncut; XbaI--4.7, 0.2.

The BstNI site at nt 8 was filled in and a HindIII linker was added. The 3' end of the insert includes the poly(A) tail, the added GC tail, and the BamHI site engineered from the original pMG-5 vector.

Classification

Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod

References

Lomedico PT, et al. Cloning and expression of murine interleukin-1 cDNA in Escherichia coli. Nature 312: 458-462, 1984. PubMed: 6209582

Ueli Gubler, personal communication

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