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Construct size (kb): 44.43000030517578

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pHC79
Intact vector size: 6.430
Type of vector: cosmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: EcoRI ClaI BamHI SalI EcaI PstI
Polylinker sites:
Construction: pBR322, Charon 4A
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, tetR
replicon: pMB1
terminator: none
Cross references: DNA Seq. Acc.: L08873

DNA: genomic

DESCRIPTION OF INSERT COMPONENT:
Genome: Saccharomyces cerevisiaeCandida robusta
Gene symbol: CUP5
Gene name: vacuolar ATP synthase, 16 kDa proteolipid subunit;
dicyclohexylcarbodiimide binding subunit
Contains complete coding sequence?: Y
Chromosome: V
Genome: Saccharomyces cerevisiaeCandida robusta
Gene symbol: RIP1
Gene name: Rieske iron-sulfur protein of the mitochondrial cytochrome
bc1 complex
Contains complete coding sequence?: Y
Chromosome: V
Genome: Saccharomyces cerevisiaeCandida robusta
Gene symbol: URA3
Gene name: orotidine-5'-phosphate decarboxylase
Contains complete coding sequence?: Y
Chromosome: V; Localization: V L, 7 cM from CEN
Genome: Saccharomyces cerevisiaeCandida robusta
Gene symbol: RPR1
Gene name: RNA component of nuclear RNase P
Contains complete coding sequence?: Y
Chromosome: V
Genome: Saccharomyces cerevisiaeCandida robusta
Gene symbol: MMS21
Contains complete coding sequence?: Y
Chromosome: V
Strain: AB972 (ATCC 76269)
Type of DNA: genomic
Insert end: MboI
Insert end: MboI
Insert size (kb): 38.0
Cross references: DNA Seq. Acc.: U18530
Nucleotides ? of the insert correspond to
nucleotides 1-38086 of U18530.
DNA Seq. Acc.: U18779
Nucleotides ? of the insert correspond to
nucleotides 31670-36872 of U18779.

Insert lengths(kb): 38.0

Gene product: Rieske iron-sulfur protein of the mitochondrial cytochrome bc1 complex [CUP5]

IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.