Features: insert detection: lacZ' marker(s): HIS3 marker(s): ampR promoter: lac promoter for in vitro transcription: T3 promoter for in vitro transcription: T7 replicon: f1 replicon: pMB1 MCS: KpnI...SacI
Applications
YI-type (integrating) shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): PvuII--4.0, 0.45; BglI--1.7, 1.7, 1.05; PstI--3.5, 0.95.
A YI-type shuttle vector with HIS3 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter system for visual detection of recombinants, promoters for transcription in vitro, and an extensive polylinker flanked by BssHII sites.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436
