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pAMY8

規(guī)格:

價格:

編號:B239740

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

pAMY8

商品貨號

B239740

Designations

pAMY8

GenBank Number

X03236

培養(yǎng)基

Species

Bacillus licheniformis (Weigmann) Chester

Depositors

T Thiel

Applications

contains sequence amylase, alpha 1A; salivary Amylase, salivary, alpha-1A

Vector

Construct size (kb): 6.300000190734863

Insert

DNA: genomic

Insert lengths(kb): 3.299999952316284

Tissue: Bacillus licheniformis

Gene product: amylase, alpha 1A; salivary(Amylase, salivary, alpha-1A) [amyE]

Target Gene: amylase, alpha 1A; salivary

Insert Size (kb)

3.300

Media

ATCC Medium 1065 (see below) plus kanamycin (50 mcg/ml) ATCC Medium 1065: Tryptone (Difco 0123), 10.0 g Yeast Extract (Difco 0127), 5.0 g NaCl, 10.0 g Distilled water, 1.0 L

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments

Restriction digests of the clone give the following sizes (kb): ClaI--4.4, 2.0; EcoRI--6.5; HindIII--3.2 (doublet).

Alpha amylase expression expressed in Escherichia coli, NPT encodes kanamycin resistance. Insert includes more than one sequenced gene.

References

Elhai J, Wolk CP. A versatile class of positive-selection vectors based on the nonviability of palindrome-containing plasmids that allows cloning into long polylinkers. Gene 68: 119-138, 1988. PubMed: 2851487

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