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pSYC1174 [CMCC 2422]
pSYC1174 [CMCC 2422]
規格:
價格:
編號:B240523
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pSYC1174 [CMCC 2422]
商品貨號
B240523
Designations
pSYC1174 [CMCC 2422]
GenBank Number

I02088

培養基
Species
Escherichia coli (Migula) Castellani and Chalmers
Depositors
Chiron Corp.
Applications
produces protein methionine aminopeptidase
Vector
Construct size (kb): 5.900000095367432
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pUC18
Intact vector size: 2.686
Type of vector: plasmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Polylinker sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Construction: pUC71K
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ', <-
MCS: HindIII...EcoRI, ->
promoter: lac, <-
replicon: pMB1, <-
marker(s): ampR, <-
Cross references: DNA Seq. Acc.: L08752
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Escherichia coli
Gene symbol: map
Gene name: methionine aminopeptidase
Contains complete coding sequence?: Y
Strain: CM89
Type of DNA: genomic
Insert end: Sau3AI
Insert end: Sau3AI
Insert size (kb): 3.2
Cross references: DNA Seq. Acc.: I02088
Insert lengths(kb): 3.200000047683716
Gene product: methionine aminopeptidase [map]
Target Gene: methionine aminopeptidase
Insert Size (kb)
3.200
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--3.4, 2.6; PstI--3.7, 2.2; SmaI--5.9.
Classification
Enterobacteriaceae, Escherichia
References

Ben-Bassat A, et al. Bacterial methionine N-terminal peptidase. US Patent 4,865,974 dated Sep 12 1989

Ben-Bassat A, et al. Processing of the initiation methionine from proteins: properties of the Escherichia coli aminopeptidase and its gene structure. J. Bacteriol. 169: 751-757, 1987. PubMed: 3027045

Ben-Bassat A, et al. Bacterial methionine N-terminal peptidase. US Patent 4,870,017 dated Sep 26 1989

Ben-Bassat A, et al. Bacterial methionine N-terminal peptidase. US Patent 5,013,662 dated May 7 1991

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
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