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12f [p12f]
12f [p12f]
規(guī)格:
價(jià)格:
編號(hào):B240664
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
12f [p12f]
商品貨號(hào)
B240664
Designations
12f [p12f]
Species
Homo sapiens, human
培養(yǎng)基
Depositors
J Weissenbach
Vector
Construct size (kb): 9.359999656677246
Insert
DNA: genomic
Insert lengths(kb): 5.0
Gene product: DNA Segment, single copy [DYS11]
Alleles: B2, B1, A1, A2
Insert Size (kb)
5.0
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
BglII digestion of recombinant plasmid gives fragments of 5.2, 2.2, and 1.9 kb.
Restriction digests of the clone give the following sizes (kb): EcoRI--5.4, 4.4; BamHI--9.8; PstI--6.2, 3.7; PvuI--9.8; BamHI/PstI--3.7, 3.4, 2.5.
The depositor indicates that the BglII fragment sizes are slightly overestimated in this reference.
EcoRI detects numerous bands, 3 of which are Y-specific. Male-specific band A (5 kb) is the cognate one and may correspond to 2 to 3 copies; maps to Yq11 and Yq12. Band B appears as 4.5 or 3 kb; maps to Yq11. Band C is about 1 kb and maps to Yq11.
TaqI detects a 10 kb band equivalent to EcoRI band B (4.5 kb allele), an 8.5 kb band equivalent to EcoRI band A and B (3.0 kb allele), and a 3.8 kb band equivalent to EcoRI fragment A.
The BglII subfragment of 2.2 kb preferentially detects the Y-specific bands.
Use stringent wash conditions (0.1X SSC, 68C).
References

Vergnaud G, et al. A deletion map of the human Y chromosome based on DNA hybridization. Am. J. Hum. Genet. 38: 109-124, 1986. PubMed: 3004206

Casanova M, et al. A human Y-linked DNA polymorphism and its potential for estimating genetic and evolutionary distance. Science 230: 1403-1406, 1985. PubMed: 2999986

Jean Weissenbach, personal communication

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