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Polytomella caeca Pringsheim
Polytomella caeca Pringsheim
規(guī)格:
價(jià)格:
編號(hào):B240701
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng)
Polytomella caeca Pringsheim
商品貨號(hào)
B240701
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format
freeze-dried
培養(yǎng)基
Type Strain
no
Comments
Preservation
Life cycle
Medium
ATCC® Medium 351: Hutner's medium for Euglena
Growth Conditions
Temperature: 25.0°C
Duration: axenic
Protocol: ATCCNO: 12910 SPEC: Aseptically add 0.5 ml of the growth medium to the inner shell vial containing the filter paper and incubate at 20C in the dark. Maintain the level of the medium in the vials at 0.5 ml during the incubation period. Observe daily for motile cells (motile cells should be observed within 6-8 days after rehydration). When numerous cells are evident, transfer the entire liquid contents of the vial (not the filter paper) to a tube of fresh medium and incubate at 25C.
Subcultivation
Protocol: ATCCNO: 12910 SPEC: Aseptically add 0.5 ml of the growth medium to the inner shell vial containing the filter paper and incubate at 20C in the dark. Maintain the level of the medium in the vials at 0.5 ml during the incubation period. Observe daily for motile cells (motile cells should be observed within 6-8 days after rehydration). When numerous cells are evident, transfer the entire liquid contents of the vial (not the filter paper) to a tube of fresh medium and incubate at 25C.
Name of Depositor
DL Wise
Chain of Custody
ATCC <<--DL Wise<<--UTEX 963 <<--- E.G. Pringsheim -- (?)
References

Reed RB, et al. Preservation of encysted Polytomella. J. Gen. Microbiol. 97: 29-34, 1976. PubMed: 186558

Lewis E, et al. Life cycle of Polytomella caeca (phytomonadida, Polyblepharidae). J. Protozool. 21: 647-649, 1974.

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