日本天天色,一级色网站,亚洲国产日韩av

SU-CCS-1

規格:

價格:

編號:B240979

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

加入購物車

在線咨詢收藏

產品名稱

SU-CCS-1

商品貨號

B240979

Organism

Homo sapiens, human

Tissue

soft tissue

培養基

Cell Type

clear cell sarcoma cell line

Product Format

frozen

Morphology

lymphoblast-like

Culture Properties

suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

clear cell sarcoma

Age

Gender

female

Ethnicity

Caucasian

Applications

A valuable tool to study EWS/ATF1 fusion gene produced by a consistent t(12;22)(q13;q12) chromosomal translocation.

Storage Conditions

liquid nitrogen vapor phase

Karyotype

t(12;22)(q13;q12) chromosomal translocation

Images

Derivation

SU-CCS-1 cell line was established from the pleural effusion of a 16-year-old Caucasian girl with clear cell sarcoma. She had been in excellent health until July 1975, when she developed a small, painful lump in her right heel. X-rays at that time were negative. The patient continued to have increased swelling and mild, intermittent right ankle pain until she was admitted to Children's Hospital at Stanford, CA, in May, 1976, with a 4-cm right inguinal mass and multiple pulmonary coin lesions. A biopsy of the right ankle mass revealed a cream-white tumor with the morphological features of clear cell sarcoma. Negative studies included a bone scan, a liver-spleen scan, a brain computer-assisted tomography scan, an i.v. pyelogram, a bone marrow aspirate and biopsy, and a lumbar puncture with spinal fluid evaluation. The patient was treated initially with pulse administration of vincristine, actinomycin D, and Cytoxan on May 17, 1976, without an objective response. In June 1976, she received one course of vincristine, Adriamycin, Cytoxan, and 5-(3,3-dimethyl-1 -triazenyl)-1 H-imidazole-4-carboxamide) chemotherapy followed by 4750 rads to the right groin for palliative treatment. She also was treated with high-dose methotrexate (300 mg/kg) with leucovorin rescue and high-dose Cytoxan (40 mg/kg/day) for 4 days again without response. In August 1976, she developed bilateral serosanguinous ma lignant pleural effusions which required Atabrine therapy. By mid-August, the patient had severe cachexia and developed congestive heart failure and pulmonary edema. Despite supportive care, she expired on September 12, 1976. Autopsy revealed extensive metastatic disease involving the lymphatics, bone marrow, lung, pleura, pericardium, liver, and a recurrent mass at the site of the primary tumor.

Clinical Data

16 years

female

Caucasian

Oncogene

EWS/ATF1 fusion gene

Tumorigenic

Yes

Comments

Clear cell sarcoma (CCS) is associated with the EWS/ATF1 oncogene that is created by aberrant t(12;22)(q13;q12) chromosomal fusion of the Ewings Sarcoma oncogene (EWS) and the cellular transcription factor ATF1. SU-CCS-1 cell line has been revealed to express the EWS/ATF1 fusion protein, and been used as a valuable tool to study CCS biology and EWS/ATF1 fusion.

SU-CCS-1 cell line was also showed to actively produce a-melanotropin, S-100 antigen, and nerve growth factor, as well as produce bombesin, The malignant potential of the established SU-CCS-1 cell line has been confirmed by the original lab. The cells were injected into nude mice by s.c. and i.e. routes of inoculation. Both methods of inoculation were found to successfully produce tumors in the nude, athymic mouse.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.

Subculturing

Culture can be maintained by the adding of fresh medium or by centrifugation and re-suspension with fresh medium when the culture becomes high density. An inoculum of 2X 10(5) to 5 x 10(5) cells/mL is recommended. Subculture when cell concentration is between 1 x 10(6) and 2 x 10(6) cells/mL.
A subcultivation ratio of 1:2 to 1:10 is recommended.
Medium renewal every 2 to 3 days.

Cryopreservation

Freeze medium: Complete growth medium supplemented with an additional 20% fetal bovine serum and 10% DMSO.

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO₂) 5%

Cells per Vial

5.0x10⁶ or more

STR Profile

Amelogenin: X
CSF1PO: 10, 11
D13S317: 8
D16S539: 11
D5S818: 12, 14
D7S820: 12
TH01: 6, 7
TPOX: 8, 11
vWA: 16, 17

Name of Depositor

Alan L. Epstein

Year of Origin

1977

References

Epstein A, et al. Use of a Newly Established Human Cell Line (SU-CCS-1) to Demonstrate the Relationship of Clear Cell Sarcoma to Malignant Melanoma. Cancer Research 44(3):1265-1274, 1984. Pubmed: 6362860

Zucman J, et al. EWS and ATF-1 gene fusion induced by t(12:22) translocation in malignant melanoma of soft parts. Nature Genetics 4(4):341-345, 1993. Pubmed: 8401579

Li KK, et al. The melanocyte inducing factor MITF is stably expressed in cell lines form human clear cell sarcoma. Br. J. Cancer 89(6):1072-1078, 2003. PubMed: 12966428

Sonobe h, et al. Further characterization of the human clear cell sarcoma cell line HS-MM demonstrating a specific t(12;22)(q13;q12) translocation and hybrid EWS/ATF-1 transcript. J.Pathol. 187(5):594-597, 1999. PubMed: 10398127

王經理

手機號：17280875617

QQ：1438578920
胡經理

手機號：13345964880

QQ：2438244627
周經理

手機號：17757487661

QQ：1296385441
于經理

手機號：18067160830

QQ：2088210172
沈經理

手機號：19548299266

QQ：2662369050
李經理

手機號：13626845108

QQ：972239479