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當前位置: 首頁 > ATCC代理 > lambdahHEX-15 B241019
lambdahHEX-15
lambdahHEX-15
規格:
價格:
編號:B241019
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
lambdahHEX-15
商品貨號
B241019
Designations
lambdahHEX-15
Species
Homo sapiens, human
培養基
Applications
A 3.25 kb EcoRI fragment of this clone was used to detect the HincII polymorphism and is the insert of clone phHK15-2 (ATCC 61512).
Vector
Construct size (kb): 46.59999847412109
Insert
DNA: cDNA
Insert lengths(kb): 3.599999904632568
Tissue: kidney
Gene product: hexokinase 1 [HK1]
Alleles: A2, A1
Insert Size (kb)
3.600
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--25.0, 15.0, 10.0, 3.5; HindIII--24.0, 10.0, 8.4, 4.6, 3.1; BamHI--20.0, 12.0, 7.0, 5.6, 4.8, 3.3, 1.9, 1.4; SalI--38.0, 13.5; XbaI--52.0.
The insert includes nucleotides 38-3598, encoding a complete 917 amino acid protein. There are 43 bp 5' untranslated and 765 bp 3' untranslated sequence in this insert.
A 3.25 kb EcoRI fragment of this clone was used to detect the HincII polymorphism and is the insert of clone phHK15-2 (ATCC 61512).
Enzyme(s) not detecting polymorphism: BclI, BglI, BglII, EcoRI, MspI, PstI, RsaI, SphI, TaqI, XbaI in a panel of 14 unrelated individuals.
References

Nishi S, et al. HincII RFLP at the human hexokinase I (HK1) locus on chromosome 10. Nucleic Acids Res. 17: 7547, 1989. PubMed: 2571978

Nishi S, et al. Human hexokinase: sequences of amino- and carboxyl-terminal halves are homologous. Biochem. Biophys. Res. Commun. 157: 937-943, 1988. PubMed: 3207429

Graeme I Bell, personal communication

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