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Human adenovirus 4

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編號:B241080

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

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凍干粉

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產品名稱

Human adenovirus 4

商品貨號

B241080

Classification

Adenoviridae, Mastadenovirus, Human adenovirus E

Agent

Human adenovirus 4

培養基

Strain

RI-67

Applications

Respiratory research

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format

frozen 1 mL per vial

Storage Conditions

-70°C or colder

Comments

Derived by adaptation of HEK cell product ATCC^® VR-1081™ to HeLa-229 cells (ATCC^® CCL-2.1™). ATCC^® VR-1081™ and ATCC^® VR-1572™ have not been compared for sequence or infectivity in primary cell culture and tissue culture.

HEK cells are known to contain Human adenovirus 5 DNA, and Human adenovirus 5 has been detected by PCR in this item.

Effect on Host

CPE, cell rounding and degeneration

Recommended Host

HeLa-229 (ATCC^® CCL-2.1™)

Growth Conditions

Temperature: 37°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 70-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm²) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO₂ atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 3-7 days

Name of Depositor

WP Rowe, National Institute of Allergy and Infectious Diseases; ATCC^®

Special Collection

ATCC

Source

Throat washings of patient, Fort Leonard Wood, Missouri, 1952-1953

References

Huebner RJ, et al. Adenoidal-pharyngeal-conjunctival agents: a newly recognized group of common viruses of the respiratory system. N. Engl. J. Med. 251: 1077-1086, 1954. PubMed: 13223958

Werner JH, Hilleman MR. Recovery of new agent from patients with acute respiratory illness. Proc. Soc. Exp. Biol. Med. 85: 183-188, 1954. PubMed: 13134329

B244801：Human adenovirus 21

B244635：Human echovirus 31

B244601：Human herpesvirus 5

B244599：DNA from Human herpesvirus 1, strain HF

B244392：Human echovirus 21

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