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AA35
AA35
規(guī)格:
價(jià)格:
編號(hào):B241167
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng)
AA35
商品貨號(hào)
B241167
Designations
AA35
GenBank Number

L05929

M91388

培養(yǎng)基
Species
Homo sapiens, human
Depositors
A Swaroop
Applications
contains sequence DNA Segment
contains sequence SEC13 (S. cerevisiae) - like 1
Vector
Construct size (kb): 4.300000190734863
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript KS-
Intact vector size: 2.964
Type of vector: phagemid
Vector end: EcoRI
Vector end: HindIII
Cloning sites: SacII XmaII NotI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV
HindIII ClaI (SalI HincII AccI) XhoI DraII ApaI KpnI
Polylinker sites: SEE COMMENTS
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
terminator: none
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: SEC13L1
Other gene symbols: D3S1231E
Gene name: SEC13 (S. cerevisiae) - like 1
Contains complete coding sequence?: Y
Chromosome: 3; Localization: 3 p25-p24
Tissue: retinal pigment epithelial cell line
Type of DNA: cDNA
Insert 5' end: Modification: EcoRI
Insert 3' end: Modification: HindIII
Insert size (kb): 1.322
Cross references: DNA Seq. Acc.: L05929
Nucleotides 45-405 of the insert correspond to
nucleotides 87-447 of L05929 with 61% similarity.
DNA Seq. Acc.: M91388
Nucleotides 1322-1085 of the insert correspond to
nucleotides 1-238 of M91388 with 100% identity.
Insert lengths(kb): 1.322000026702881
Tissue: retinal pigment epithelial cell line
Gene product: SEC13 (S. cerevisiae) - like 1 [SEC13L1]
Target Gene: DNA Segment, SEC13 (S. cerevisiae) - like 1
Insert Size (kb)
1.322
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--3.0, 1.3; PvuII--2.5, 0.7, 0.5, 0.45, 0.2; SacI--3.8, 0.5; SalI--3.1, 1.2; SphI--4.3.
The insert contains the following restriction sites (approximate kb from the 5' end): BspHI--0.18; HincII--0.16; PvuII--0.48, 0.71, 1.17; SacI--0.38; SalI--0.16; SphI--0.31.
Detects a related sequence on human chromosome 18 q11.2-q12.
References

Swaroop A, et al. Molecular characterization of a novel human gene, SEC13R, related to the yeast secretory pathway gene SEC13, and mapping to a conserved linkage group on human chromosome 3p24-p25 and mouse chromosome 6. Hum. Mol. Genet. 3: 1281-1286, 1994. PubMed: 7987303

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