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CRI-O84
CRI-O84
規格:
價格:
編號:B241380
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
CRI-O84
商品貨號
B241380
Designations
CRI-O84
Species
Homo sapiens, human
培養基
Depositors
Collaborative Research, Inc.
Vector
Construct size (kb): 46.0
Insert
DNA: genomic
Insert lengths(kb): 40.0
Gene product: DNA Segment, single copy [D16S43]
Alleles: B1, B1, B2, B2, B3, B4, A1, A2, B3, B4
Insert Size (kb)
40.0
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--19.0, 8.0, 5.5, 4.5, 2.5, 2.4, 2.2, 1.9; BamHI--17.0, 11.0, 7.0, 6.4, 3.1, 1.9; HincII--8.4 (doublet), 6.5, 3.3 (doublet), 3.0, 1.8, 1.75, 1.65, 1.4, 1.3;
HindIII--11.0, 6.5, 6.0, 5.4, 4.0 (doublet), 3.5, 2.1, 1.8; BglII--17.0 (doublet), 3.0 (doublet), 2.6.
The insert can be excised from the vector with EcoRI, resulting in a 5.5 kb EcoRI vector fragment.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
The PstI and HindIII polymorphisms have a combined PIC value of 0.79 and a combined heterozygosity of 83%.
The HindIII polymorphisms have a combined heterozygosity of 33%.
Enzyme(s) not detecting polymorphism: BglII, EcoRI, MspI (panel of 5 individuals).
References

Donis-Keller H, et al. A genetic linkage map of the huma genome. Cell 51: 319-337, 1987. PubMed: 3664638

Keith TP, et al. Genetic linkage map of 43 DNA markers on human chromosome 16. Proc. Natl. Acad. Sci. USA 87: 5754-5758, 1990. PubMed: 2377614

Jacobsen SJ, et al. A clinically homogeneous group of families with facioscapulohumeral (Landouzy-Dejerine) muscular dystrophy: linkage analysis of six autosomes. Am. J. Hum. Genet. 47: 376-388, 1990. PubMed: 1975474

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